Coding

Part:BBa_K3755015:Design

Designed by: Kaijun Wang   Group: iGEM21_ShanghaiTech_China   (2021-10-01)


mRuby2 red fluorescent protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 16


Design Notes

None


Source

This red fluorescent protein is discover by screening a library of mutant mRuby. We got a plasmid containing this protein from Bailong Xiao's lab, Tsinghua university.

References

[1]Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin MZ. Improving FRET dynamic range with bright green and red fluorescent proteins. Nat Methods. 2012 Oct;9(10):1005-12. doi: 10.1038/nmeth.2171. Epub 2012 Sep 9. PMID: 22961245; PMCID: PMC3461113.